rabbit anti lyve1  (Novus Biologicals)

Journal: Frontiers in Pediatrics

Article Title: PROX1-associated lymphatic reprogramming signatures in pediatric adamantinomatous craniopharyngioma: a comparative study with adult cases

doi: 10.3389/fped.2026.1865684

Figure Lengend Snippet: Spatial distribution and co-expression of endothelial and lymphatic markers in pediatric ACP tissues. Representative IHC staining of serial sections from pediatric ACP tissues demonstrating the spatial distribution of CD34, PROX1, and LYVE1. All three markers display overlapping expression that clearly delineates the same ring-like structures, indicating a distinct lymphatic phenotype within the tumor microenvironment. Scale bar, 50 µm.

Article Snippet: For IHC, primary antibodies against PROX1 (Rabbit mAb, ABclonal, Wuhan, China), Nestin (Rabbit mAb, ABclonal), CD34 (Rabbit PolymAb, ABclonal), and LYVE1 (Rabbit mAb, ABclonal) were employed, followed by incubation with appropriate secondary antibodies (Zhongshan Golden Bridge Biotechnology, Beijing, China).

Techniques: Expressing, Immunohistochemistry

Journal: Frontiers in Pediatrics

Article Title: PROX1-associated lymphatic reprogramming signatures in pediatric adamantinomatous craniopharyngioma: a comparative study with adult cases

doi: 10.3389/fped.2026.1865684

Figure Lengend Snippet: Comparative analysis of PROX1 and LYVE1 expression in children versus adult ACP. (A) Representative IHC staining delineating the spatial distribution of PROX1 and LYVE1 in pediatric and adult ACP tissues. (B) Quantitative analysis showing the differential expression of PROX1 ( n = 6) and LYVE1 ( n = 5) between pediatric and adult cohorts. Scale bar, 50 µm. *: P < 0.05; **: P < 0.01.

Article Snippet: For IHC, primary antibodies against PROX1 (Rabbit mAb, ABclonal, Wuhan, China), Nestin (Rabbit mAb, ABclonal), CD34 (Rabbit PolymAb, ABclonal), and LYVE1 (Rabbit mAb, ABclonal) were employed, followed by incubation with appropriate secondary antibodies (Zhongshan Golden Bridge Biotechnology, Beijing, China).

Techniques: Analysis, Expressing, Immunohistochemistry, Quantitative Proteomics

Journal: Translational Stroke Research

Article Title: CircRNA circ_0004058 Modulates Early Brain Injury in Subarachnoid Hemorrhage Through miR-221-3p and VE1 Activation Pathway

doi: 10.1007/s12975-025-01383-9

Figure Lengend Snippet: Identification of key genes involved in SAH onset and validation in vivo. A : Volcano plot of differentially expressed genes between samples of brain tissue from SAH (n = 10) and sham (n = 10) rats according to RNA sequencing; red represents upregulated genes and green represents downregulated genes. B : Venn diagram of differentially expressed genes according to RNA sequencing and SAH-related genes analyzed by GeneCards. C–D : Expression levels of LYVE1 (C) and TEK (D) extracted from RNA sequencing data. E–F : mRNA and protein levels of LYVE1 in the hippocampal tissue of sham-operated and SAH rats, as detected by RT-qPCR (E) and Western blotting (F). G : Flow cytometry detection of LYVE1 + CD68 + macrophages in the hippocampal tissue of sham-operated and SAH rats. H–I : mRNA and protein levels of VEGF-A in the hippocampal tissue of sham-operated and SAH rats, as detected by RT-qPCR (H) and Western blotting (I). (J) Immunohistochemistry detection of CD31 and VEGFA protein levels in the hippocampal tissue of each group of rats. n = 8 rats per treatment. *** p < 0.001 vs. sham-operated rats

Article Snippet: After blocking with 5% bovine serum albumin, membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies against LYVE1 (1:1000, #67,538, Cell Signaling Technology), vascular endothelial growth factor (VEGF)-A (1:1000, ab214424, Abcam), and GAPDH (ab181602, 1:10,000, Abcam).

Techniques: Biomarker Discovery, In Vivo, RNA Sequencing, Expressing, Quantitative RT-PCR, Western Blot, Flow Cytometry, Immunohistochemistry

Journal: Translational Stroke Research

Article Title: CircRNA circ_0004058 Modulates Early Brain Injury in Subarachnoid Hemorrhage Through miR-221-3p and VE1 Activation Pathway

doi: 10.1007/s12975-025-01383-9

Figure Lengend Snippet: Effects of LYVE1 overexpression on brain injury in SAH rats. ote: SAH rats were treated with oe-NC or oe-LYVE1. A : RT-qPCR detection of LYVE1 mRNA expression in the hippocampal tissue of SAH rats. B : Western blot of LYVE1 protein expression in the hippocampal tissue of SAH rats. C–D : Neurological function scores (C) and extent of cerebral edema (D) in SAH rats. E : The pathological scores of SAH rats. F : Representative images of brain specimens collected after SAH induction and quantitative assessment of SAH severity; G : Evans blue staining was used to evaluate BBB permeability and quantify Evans blue dye extravasation in each group; scale bar = 0.5 cm; H : Nissl staining of neuronal injury in the hippocampal tissue of SAH rats. I : TUNEL staining of neuronal apoptosis in the hippocampal tissue of SAH rats. J : Flow cytometry analysis of LYVE1 + CD68 + macrophages in the hippocampal tissue of SAH rats. K–L : mRNA and protein levels of VEGF-A in the hippocampal tissue of SAH rats, as detected by RT-qPCR (K) and Western blotting (L); M: Immunohistochemistry was used to detect CD31 and VEGFA protein levels in the hippocampal tissue of each group. n = 8 rats per treatment. ** p < 0.01, *** p < 0.001vs. treatment with oe-N

Article Snippet: After blocking with 5% bovine serum albumin, membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies against LYVE1 (1:1000, #67,538, Cell Signaling Technology), vascular endothelial growth factor (VEGF)-A (1:1000, ab214424, Abcam), and GAPDH (ab181602, 1:10,000, Abcam).

Techniques: Over Expression, Quantitative RT-PCR, Expressing, Western Blot, Staining, Permeability, TUNEL Assay, Flow Cytometry, Immunohistochemistry

Journal: Translational Stroke Research

Article Title: CircRNA circ_0004058 Modulates Early Brain Injury in Subarachnoid Hemorrhage Through miR-221-3p and VE1 Activation Pathway

doi: 10.1007/s12975-025-01383-9

Figure Lengend Snippet: Identification of miRNAs upstream of LYVE1. A : Volcano plot of differentially expressed miRNAs between control (n = 2) and SAH (n = 2) samples in the GSE161870 dataset. B: Venn diagram of regulatory miRNAs upstream of LYVE1, as predicted by miRWalk database, and differentially expressed miRNAs in the GSE161870 dataset. C : Heat map of the expression of candidate miRNAs in the GSE161870 dataset. D : miR-221-3p expression in the hippocampal tissue of sham-operated and SAH rats, as detected by RT-qPCR. E : miR-221-3p binding sites on LYVE1 mRNA, as predicted by miRWalk database. F : miR-221-3p binding to LYVE1, as confirmed by dual-luciferase reporter assay. G : Expression levels of miR-221-3p and LYVE1 in RMA-BMs co-cultured with primary rat hippocampal neurons that had been transfected with miR-221-3p mimic or miR-221-3p inhibitor and then treated with GW4869, as detected by RT-qPCR. H : Western blot of LYVE1 protein expression in RMA-BMs co-cultured with primary rat hippocampal neurons that had been transfected with miR-221-3p mimic or miR-221-3p inhibitor and then treated with GW4869. n = 8 rats per treatment. *** p < 0.001 vs. treatment with NC mimic or NC inhibitor. Cell experiments were repeated three times

Article Snippet: After blocking with 5% bovine serum albumin, membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies against LYVE1 (1:1000, #67,538, Cell Signaling Technology), vascular endothelial growth factor (VEGF)-A (1:1000, ab214424, Abcam), and GAPDH (ab181602, 1:10,000, Abcam).

Techniques: Control, Expressing, Quantitative RT-PCR, Binding Assay, Luciferase, Reporter Assay, Cell Culture, Transfection, Western Blot

Journal: Translational Stroke Research

Article Title: CircRNA circ_0004058 Modulates Early Brain Injury in Subarachnoid Hemorrhage Through miR-221-3p and VE1 Activation Pathway

doi: 10.1007/s12975-025-01383-9

Figure Lengend Snippet: Effects of miR-221-3p-mediated regulation of LYVE1 on EBI in SAH rats. Note: SAH rats were treated with miR-221-3p inhibitor alone or in combination with sh-LYVE1. A : RT-qPCR detection of miR-221-3p expression and LYVE1 mRNA expression in the hippocampal tissue of SAH rats. B : Western blot of LYVE1 protein expression in the hippocampal tissue of SAH rats. C – D : Neurological function scores (C) and extent of cerebral edema (D) in SAH rats. E : The pathological scores of SAH rats; F : Representative images of brain specimens collected after SAH induction and quantitative assessment of SAH severity. G : Evans blue staining to assess BBB permeability and quantify Evans blue dye extravasation in each group; scale bar = 0.5 cm; H : Nissl staining of neuronal injury in the hippocampal tissue of SAH rats. I : TUNEL staining of neuronal apoptosis in the hippocampal tissue of SAH rats. J : Flow cytometry analysis of LYVE1 + CD68. + macrophages in the hippocampal tissue of SAH rats. K–L : mRNA and protein levels of VEGF-A in the hippocampal tissue of SAH rats, as detected by RT-qPCR (K) and Western blotting (L). M : Immunohistochemistry to detect CD31 and VEGFA protein levels in the hippocampal tissue of each group. n = 8 rats per treatment. *** p < 0.001

Article Snippet: After blocking with 5% bovine serum albumin, membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies against LYVE1 (1:1000, #67,538, Cell Signaling Technology), vascular endothelial growth factor (VEGF)-A (1:1000, ab214424, Abcam), and GAPDH (ab181602, 1:10,000, Abcam).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Staining, Permeability, TUNEL Assay, Flow Cytometry, Immunohistochemistry

Journal: Translational Stroke Research

Article Title: CircRNA circ_0004058 Modulates Early Brain Injury in Subarachnoid Hemorrhage Through miR-221-3p and VE1 Activation Pathway

doi: 10.1007/s12975-025-01383-9

Figure Lengend Snippet: Relationship among circ_0004058, miR-221-3p, and LYVE1. A : Volcano plot of differentially expressed circRNAs between control (n = 4) and SAH (n = 5) samples in the GSE161913 dataset. B : Venn diagram of regulatory circRNAs upstream of miR-221-3p, as predicted by circBank database, and differentially expressed circRNAs in the GSE161913 dataset. C : Differential expression analysis of circ_0000826 and circ_0004058 in the GSE161913 dataset (Control, n = 4; SAH, n = 5). D : Stability of circ_0004058, as determined by RNase R digestion. E : circ_0004058 expression in the hippocampal tissue of sham-operated and SAH rats, as detected by RT-qPCR. F : circ_0004058 and miR-221-3p binding sites, as predicted by starBase database. G : Binding of circ_0004058 to miR-221-3p, as confirmed by dual-luciferase reporter assay. H : circ_0004058 binding to miR-221-3p, as detected by RNA pull-down assays. I : circ_0004058 binding to miR-221-3p, assessed by RIP assays. J : Localization of circ_0004058 and miR-221-3p in primary rat hippocampal neurons, as detected by FISH assays. K : Expression levels of miR-221-3p and LYVE1 in RMA-BMs co-cultured with primary rat hippocampal neurons that had been transfected with oe-circ_0004058 or sh-circ_0004058 and then treated with GW4869, as detected by RT-qPCR. L : Western blot of LYVE1 protein expression in RMA-BMs co-cultured with primary rat hippocampal neurons that had been transfected with oe-circ_0004058 or sh-circ_0004058 and then treated with GW4869. n = 8 rats per treatment. ** p < 0.01, *** p < 0.001 vs. treatment with oe-NC or sh-NC. Cell experiments were repeated three times

Article Snippet: After blocking with 5% bovine serum albumin, membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies against LYVE1 (1:1000, #67,538, Cell Signaling Technology), vascular endothelial growth factor (VEGF)-A (1:1000, ab214424, Abcam), and GAPDH (ab181602, 1:10,000, Abcam).

Techniques: Control, Quantitative Proteomics, Expressing, Quantitative RT-PCR, Binding Assay, Luciferase, Reporter Assay, Cell Culture, Transfection, Western Blot

Journal: Translational Stroke Research

Article Title: CircRNA circ_0004058 Modulates Early Brain Injury in Subarachnoid Hemorrhage Through miR-221-3p and VE1 Activation Pathway

doi: 10.1007/s12975-025-01383-9

Figure Lengend Snippet: Effects of circ_0004058-mediated regulation of miR-221-3p and reduced EBI in SAH rats. Note: SAH rats were treated with oe-circ_0004058 alone or in combination with miR-221-3p mimic. A: RT-qPCR detection of circ_0004058, miR-221-3p, and LYVE1 expression in the hippocampal tissue of SAH rats. B: Western blot of LYVE1 protein expression in the hippocampal tissue of SAH rats. C–D: Neurological function scores (C) and extent of cerebral edema (D) in SAH rats. E: The pathological scores of SAH rats. F: Representative images of brain specimens collected after SAH induction and quantitative assessment of SAH severity. G: Evans blue staining to evaluate BBB permeability and quantitative analysis of Evans blue dye extravasation in each group; scale bar = 0.5 cm. H: Nissl staining of neuronal injury in the hippocampal tissue of SAH rats. I: TUNEL staining of neuronal apoptosis in the hippocampal tissue of SAH rats. J: Flow cytometry analysis of LYVE1 + CD68 + macrophages in the hippocampal tissue of SAH rats. K–L: mRNA and protein levels of VEGF-A in the hippocampal tissue of SAH rats, as detected by RT-qPCR (K) and Western blotting (L). M: Immunohistochemistry to detect CD31 and VEGFA protein levels in the hippocampal tissue of each group. n = 8 rats per treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. treatment with oe-NC + NC mimic or oe-circ_0004058 + NC mimic

Article Snippet: After blocking with 5% bovine serum albumin, membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies against LYVE1 (1:1000, #67,538, Cell Signaling Technology), vascular endothelial growth factor (VEGF)-A (1:1000, ab214424, Abcam), and GAPDH (ab181602, 1:10,000, Abcam).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Staining, Permeability, TUNEL Assay, Flow Cytometry, Immunohistochemistry

Journal: Translational Stroke Research

Article Title: CircRNA circ_0004058 Modulates Early Brain Injury in Subarachnoid Hemorrhage Through miR-221-3p and VE1 Activation Pathway

doi: 10.1007/s12975-025-01383-9

Figure Lengend Snippet: Effects of the circ_0004058/miR-221-3p/LYVE1 axis on EBI in SAH rats. Note: SAH rats were treated with oe-circ_0004058 alone or in combination with sh-LYVE1. A: RT-qPCR detection of circ_0004058, miR-221-3p, and LYVE1 expression in the hippocampal tissue of SAH rats. B: Western blot of LYVE1 protein expression in the hippocampal tissue of SAH rats. C–D: Neurological function scores (C) and extent of cerebral edema (D) in SAH rats. E: The pathological scores of SAH rats. F: Representative images of brain specimens collected after SAH induction and quantitative assessment of SAH severity. G: Evans blue staining to evaluate BBB permeability and quantitative analysis of Evans blue dye extravasation in each group; scale bar = 0.5 cm. H: Nissl staining of neuronal injury in the hippocampal tissue of SAH rats. I: TUNEL staining of neuronal apoptosis in the hippocampal tissue of SAH rats. J: Flow cytometry analysis of LYVE1 + CD68 + macrophages in the hippocampal tissue of SAH rats. K–L: mRNA and protein levels of VEGF-A in the hippocampal tissue of SAH rats, as detected by RT-qPCR (K) and Western blotting (L). M: Immunohistochemistry to detect CD31 and VEGFA protein levels in the hippocampal tissue of each group. n = 8 rats per treatment. *** p < 0.001 vs. treatment with oe-NC + sh-NC; # p < 0.05 vs. treatment with oe-circ_0004058 + sh-NC

Article Snippet: After blocking with 5% bovine serum albumin, membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies against LYVE1 (1:1000, #67,538, Cell Signaling Technology), vascular endothelial growth factor (VEGF)-A (1:1000, ab214424, Abcam), and GAPDH (ab181602, 1:10,000, Abcam).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Staining, Permeability, TUNEL Assay, Flow Cytometry, Immunohistochemistry

Journal: Translational Stroke Research

Article Title: CircRNA circ_0004058 Modulates Early Brain Injury in Subarachnoid Hemorrhage Through miR-221-3p and VE1 Activation Pathway

doi: 10.1007/s12975-025-01383-9

Figure Lengend Snippet: Effects of LYVE1 + CD68 + macrophages on EBI in SAH rats. Note: SAH rats were treated with oe-LYVE1 alone or in combination with anti-CSF1R antibody. A: RT-qPCR detection of circ_0004058, miR-221-3p, and LYVE1 expression in the hippocampal tissue of SAH rats. B: Western blot of LYVE1 protein expression in the hippocampal tissue of SAH rats. C–D: Neurological function scores (C) and extent of cerebral edema (D) in SAH rats. E: The pathological scores of SAH rats. F: Representative images of brain specimens collected after SAH induction and quantitative assessment of SAH severity. G: Evans blue staining to assess BBB permeability and quantitative analysis of Evans blue dye extravasation in each group; scale bar = 0.5 cm. H: Nissl staining of neuronal injury in the hippocampal tissue of SAH rats. I: TUNEL staining of neuronal apoptosis in the hippocampal tissue of SAH rats. J: Flow cytometry analysis of LYVE1 + CD68 + macrophages in the hippocampal tissue of SAH rats. K–L: mRNA and protein levels of VEGF-A in the hippocampal tissue of SAH rats, as detected by RT-qPCR (K) and Western blotting (L). n = 8 rats per treatment. M: Immunohistochemistry to detect CD31 and VEGFA protein levels in the hippocampal tissue of each group. ** p < 0.01, *** p < 0.001 vs. treatment with oe-NC + anti-IgG; # p < 0.05 vs. treatment with oe-LYVE1 + anti-IgG

Article Snippet: After blocking with 5% bovine serum albumin, membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies against LYVE1 (1:1000, #67,538, Cell Signaling Technology), vascular endothelial growth factor (VEGF)-A (1:1000, ab214424, Abcam), and GAPDH (ab181602, 1:10,000, Abcam).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Staining, Permeability, TUNEL Assay, Flow Cytometry, Immunohistochemistry

Journal: Translational Stroke Research

Article Title: CircRNA circ_0004058 Modulates Early Brain Injury in Subarachnoid Hemorrhage Through miR-221-3p and VE1 Activation Pathway

doi: 10.1007/s12975-025-01383-9

Figure Lengend Snippet: Schematic diagram of the mechanism by which the circ_0004058/miR-221-3p/LYVE1 axis affects SAH-induced EBI

Article Snippet: After blocking with 5% bovine serum albumin, membranes were incubated overnight at 4 °C with rabbit monoclonal antibodies against LYVE1 (1:1000, #67,538, Cell Signaling Technology), vascular endothelial growth factor (VEGF)-A (1:1000, ab214424, Abcam), and GAPDH (ab181602, 1:10,000, Abcam).

Techniques: